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Dr Andrei Mardaryev

PositionLecturer in Stem Cell Biology and Regenerative Medicine
LocationH38F, Richmond Building
DepartmentSchool of Chemistry and Bioscience
RKT CentreCentre for Skin Sciences
Telephone01274 234732
Emaila.mardaryev@bradford.ac.uk
ORCID0000-0002-7826-5506

Research Interests (key words only)

Skin, hair follicle, stem cells, chromatin, epigenetics, wound healing

PhD Supervision

  • Ms Inemo Asamaowei (current)
  • Mr David Broadley (current)
  • Ms Serena Buscone (completed 2017)
  • Mr Igor Malashchuk (completed 2016)
  • Ms Valentina Rapisarda (completed 2014)

Teaching and Supervisory Responsibilities

Administrative Responsibilities

  • Manager for FACS and cell sorting facility at the Centre for Skin Sciences (University of Bradford)
  • Academic admission team
  • Programme team member for development MSc in Skin Biology & Regenerative Medicine
  • Programme team member for undergraduate medical curriculum design

Study History

  • Chuvash State University, School of Medicine, Russia (MD, 2003) 
  • University of Bradford (PhD in Medical Biosciences, 2009)
  • University of Bradford (Postgraduate Certificate in Higher Education, 2017)

Professional Activities

Member of The European Society for Dermatology Research (ESDR)

Reviewer for Leading Life Science Journals:-

  • PLOS Genetics
  • Journal of Investigative Dermatology
  • PLOS One
  • Experimental Dermatology
  • Stem Cell Report
  • Scientific Reports

Research Areas

Gene expression is regulated on multiple levels that include a) direct binding of transcriptional factors to the regulatory regions of genes; b) dynamic compaction of linear DNA around histone proteins; c) higher-order chromatin folding. One of the greatest challenges in modern molecular biology is an understanding how all these levels are integrated in complex and very well orchestrated system allowing a cell to adopt to its rapidly changing environment. I am particular interested, i) how in multipotent hair follicle stem cells intracellular signalling pathways and corresponding transcriptional network, initiated by external and intrinsic stimuli, control cell fate decision along several cell lineages; ii) how this process is correlated with changes in DNA/histone modifications and higher-order chromatin structure in tissue-specific gene loci; and finally iii) how all of these is affected in pathological conditions. The answering of these questions should advance the systemic view on hair follicle stem cells biology and will be also beneficial for development of new therapeutic approaches for stem cell-dependent conditions, such as tumorigenesis, impaired wound healing, hair loss, and aging.    

My projects are in two key areas:

  • Signalling networks, chromatin dynamics and gene regulation in epithelial stem cell biology
  • Molecular mechanisms of skin regeneration, carcinogenesis and aging

My funding is from the MRC - Young Investigator award, 2016-2019.

Current Projects

  • Role of polycomb group proteins in the control of tissue-specific gene expression programmes in skin stem cells during epidermal differentiation, hair growth and wound healing
  • Chromatin organization by CTCF in epidermal and hair follicle homeostasis

Research Collaborations

  • Prof Terumi Kohwi-Shigematsu (University of California San Francisco, USA)
  • Prof Thomas Magin (University of Leipzig, Germany)
  • Dr Andrey Sharov (Boston University, USA)
  • Prof Gualing Xu (Shanghai Institute of Biotechnology, China)

Publications

See Dr. Mardaryev's publications on Bradford Scholars.

Poterlowicz K, Yarker JL, Malashchuk I, Lajoie BR, Mardaryev AN, Gdula MR, Sharov AA, Kohwi-Shigematsu T, Botchkarev VA, Fessing MY. 5C analysis of the Epidermal Differentiation Complex locus reveals distinct chromatin interaction networks between gene-rich and gene-poor TADs in skin epithelial cells. PLoS Genet. 2017 Sep 1;13(9):e1006966.

Aunin E, Broadley D, Ahmed MI, Mardaryev AN, Botchkareva NV. Exploring a Role for Regulatory miRNAs In Wound Healing during Ageing: Involvement of miR-200c in wound repair. Sci Rep. 2017 Jun 12;7(1):3257. doi: 10.1038/s41598-017-03331-6.

Rapisarda V, Malashchuk I, Asamaowei IE, Poterlowicz K, Fessing MY, Sharov AA, Karakesisoglou I, Botchkarev VA, Mardaryev A. p63 transcription factor regulates nuclear shape and expression of nuclear envelope-associated genes in epidermal keratinocytes. J Invest Dermatol. 2017 Jun 5. pii: S0022-202X(17)31553-1. doi: 10.1016/j.jid.2017.05.013. [Epub ahead of print]

Buscone S, Mardaryev AN, Raafs B, Bikker JW, Sticht C, Gretz N, Farjo N, Uzunbajakava NE, Botchkareva NV. A new path in defining light parameters for hair growth: Discovery and modulation of photoreceptors in human hair follicle. Lasers Surg Med. 2017 Apr 18. doi: 10.1002/lsm.22673. [Epub ahead of print]

Botchkarev VA, Mardaryev AN. Repressing the Keratinocyte Genome: How the Polycomb Complex Subunits Operate in Concert to Control Skin and Hair Follicle Development. J Invest Dermatol. 2016 Aug;136(8):1538-40

Mardaryev AN, Liu B, Rapisarda V, Poterlowicz K, Malashchuk I, Rudolf J, Sharov AA, Jahoda CA, Fessing MY, Benitah SA, Xu GL, Botchkarev VA. Cbx4 maintains the epithelial lineage identity and cell proliferation in the developing stratified epithelium. J Cell Biol. 2016 Jan 4;212(1):77-89

Ahmed MI, Alam M, Emelianov VU, Poterlowicz K, Patel A, Sharov AA, Mardaryev AN, Botchkareva NV. MicroRNA-214 controls skin and hair follicle development by modulating the activity of Wnt pathway. J Cell Biol. 2014;207(4):549-67

Mardaryev AN, Gdula M, Yarker J, Emelianov VN, Poterlowicz K, Sharov AA, Sharova TY, Scarpa JA, Chambon P, Botchkarev VA, Fessing MY. p63 and Brg1 control developmentally regulated higher-order chromatin remodelling at the epidermal differentiation complex locus in epidermal progenitor cells. Development. 2014;141(1):101-11

Lewis CJ, Mardaryev AN, Sharov AA, Fessing MY, Botchkarev VA. The Epigenetic Regulation of Wound Healing. Adv Wound Care (New Rochelle). 2014;3(7):468-475

Lewis CJ, Mardaryev AN, Poterlowicz K, Sharova TY, Aziz A, Sharpe DT, Botchkareva NV, Sharov AA. Bone Morphogenetic Protein Signaling Suppresses Wound-Induced Skin Repair by Inhibiting Keratinocyte Proliferation and Migration. J Invest Dermatol. 2014,134(3):827-37

Gdula MR, Poterlowicz K, Mardaryev AN, Sharov AA, Peng Y, Fessing MY, Botchkarev VA. Remodelling of Three-Dimensional Organization of the Nucleus During Terminal Keratinocyte Differentiation in the Epidermis. J Invest Dermatol. 2013;133(9):2191-201

Liu B, Liu YF, Du YR, Mardaryev AN, Yang W, Chen H, Xu ZM, Xu CQ, Zhang XR, Botchkarev VA, Zhang Y, Xu GL. Cbx4 regulates the proliferation of thymic epithelial cells and thymus function. Development. 2013;140(4):780-8

Botchkarev VA, Gdula MR, Mardaryev AN, Sharov AA, Fessing MY. Epigenetic Mechanisms of Regulation of Gene Expression in Keratinocytes. J Invest Dermatol. 2012;132(11):2505-21

Mardaryev AN, Meier N, Poterlowicz K, Sharov AA, Sharova TY, Ahmed MI, Rapisarda V, Lewis C, Fessing MY, Ruenger TM, Bhawan J, Werner S, Paus R, Botchkarev VA. Lhx2 differentially regulates Sox9, Tcf4 and Lgr5 in hair follicle stem cells to promote epidermal regeneration after injury. Development. 2011;138(22):4843-52

Fessing MY/Mardaryev AN, Gdula M, SharovAA, Sharova TY, Rapisarda V, Gordon KB, Smorodchenko AD, Poterlowicz KD, Ferone G, Kohwi Y, Missero C, Kohwi-Shigematsu T, Botchkarev VA. p63 Regulates Satb1 to Control Tissue-Specific Chromatin Remodeling during Develo

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